产品货号：
GS4375
中文名称：
重组人PEDF
英文名称：
Recombinant Human Pigment Epithelium-derived Factor
产品规格：
5μg
发货周期：
1～3天
产品价格：
询价
General Description
Pigment epithelium-derived factor (PEDF) is encoded by the SERPINF1 gene in humans and found in verebrates.It is a secreted phosphoglycoprotein that belongs to the clade F subfamily,serpin superfamily of proteinase inhibitors.The PEDF is a noninhibitory serpin with neurotrophic,anti-angiogenic,and anti-tumorigenic properties.It is synthesized as a 418 a.a.about 50kDa precursor that contains a 19 a.a.signal sequence and a 399 a.a.mature region that shows a pyroglutamate at Gln20.Like other serpins,it contains three β-sheets,810 α-helices,and a C-terminal RCL (reactive center loop).Unlike other serpins with Ser protease inhibiting activity.PEDF has functions of inducing extensive neuronal differentiation in retinoblastoma cells,inhibiting of angiogenesis.As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins,it exhibits no serine protease inhibitory activity.PEDF is researched as a therapeutic candidate for treatment of such conditions as choroidal neovascularization,heart disease,and cancer.
Storage
This lyophilized preparation is stable at 2～8℃,but should be kept at -20℃ for long term storage,preferably desiccated.Upon reconstitution,the preparation is stable for up to one week at 2～8℃.For maximal stability,apportion the reconstituted preparation into working aliquots and store at -20℃ to -70℃.Avoid repeated freeze/thaw cycles.
Usage
This material is offered by bjbalb Biotech for research,laboratory or further evaluation purposes.
NOT FOR HUMAN USE.
Molecular Weight
Approximately 44.4 KDa,a single non-glycosylated polypeptide chain containing 399 amino acids.
Purity
> 97% by SDS-PAGE and HPLC analyses.
Source
Escherichia coli.
Biological Activity
Fully biologically active when compared to standard.The ED50 as determined by its ability to enhance the adhesion of human Saos2 cells to bovine Collagen I coated plate is less than 2ng/mL,corresponding to a specific activity of> 5.0×105 IU/mg.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2μm filtered concentrated solution in 20mM PB,pH7.4,150mM NaCl.
Endotoxin
Less than 1 EU/μg of rhPEDF as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1～1.0mg/mL.Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃.
Further dilutions should be made in appropriate buffered solutions.
AA Sequence
QNPASPPEEG SPDPDSTGAL VEEEDPFFKV PVNKLAAAVS NFGYDLYRVR SSTSPTTNVL LSPLSVATAL
SALSLGAEQR TESIIHRALY YDLISSPDIH GTYKELLDTV TAPQKNLKSA SRIVFEKKLR IKSSFVAPLE
KSYGTRPRVL TGNPRLDLQE INNWVQAQMK GKLARSTKEI PDEISILLLG VAHFKGQWVT KFDSRKTSLE
DFYLDEERTV RVPMMSDPKA VL
相关搜索：重组人PEDF，rhPEDF
Pigment epithelium-derived factor (PEDF) is encoded by the SERPINF1 gene in humans and found in verebrates.It is a secreted phosphoglycoprotein that belongs to the clade F subfamily,serpin superfamily of proteinase inhibitors.The PEDF is a noninhibitory serpin with neurotrophic,anti-angiogenic,and anti-tumorigenic properties.It is synthesized as a 418 a.a.about 50kDa precursor that contains a 19 a.a.signal sequence and a 399 a.a.mature region that shows a pyroglutamate at Gln20.Like other serpins,it contains three β-sheets,810 α-helices,and a C-terminal RCL (reactive center loop).Unlike other serpins with Ser protease inhibiting activity.PEDF has functions of inducing extensive neuronal differentiation in retinoblastoma cells,inhibiting of angiogenesis.As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins,it exhibits no serine protease inhibitory activity.PEDF is researched as a therapeutic candidate for treatment of such conditions as choroidal neovascularization,heart disease,and cancer.
Storage
This lyophilized preparation is stable at 2～8℃,but should be kept at -20℃ for long term storage,preferably desiccated.Upon reconstitution,the preparation is stable for up to one week at 2～8℃.For maximal stability,apportion the reconstituted preparation into working aliquots and store at -20℃ to -70℃.Avoid repeated freeze/thaw cycles.
Usage
This material is offered by bjbalb Biotech for research,laboratory or further evaluation purposes.
NOT FOR HUMAN USE.
Molecular Weight
Approximately 44.4 KDa,a single non-glycosylated polypeptide chain containing 399 amino acids.
Purity
> 97% by SDS-PAGE and HPLC analyses.
Source
Escherichia coli.
Biological Activity
Fully biologically active when compared to standard.The ED50 as determined by its ability to enhance the adhesion of human Saos2 cells to bovine Collagen I coated plate is less than 2ng/mL,corresponding to a specific activity of> 5.0×105 IU/mg.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2μm filtered concentrated solution in 20mM PB,pH7.4,150mM NaCl.
Endotoxin
Less than 1 EU/μg of rhPEDF as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1～1.0mg/mL.Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃.
Further dilutions should be made in appropriate buffered solutions.
AA Sequence
QNPASPPEEG SPDPDSTGAL VEEEDPFFKV PVNKLAAAVS NFGYDLYRVR SSTSPTTNVL LSPLSVATAL
SALSLGAEQR TESIIHRALY YDLISSPDIH GTYKELLDTV TAPQKNLKSA SRIVFEKKLR IKSSFVAPLE
KSYGTRPRVL TGNPRLDLQE INNWVQAQMK GKLARSTKEI PDEISILLLG VAHFKGQWVT KFDSRKTSLE
DFYLDEERTV RVPMMSDPKA VL
相关搜索：重组人PEDF，rhPEDF